“Hodges Test Peptide Mix”
(from Synthetic Peptides, Inc.)
Advantage HIGHLOAD C18, 5µm 150 x 4.6mm
The aromatic hydrocarbons that most HPLC column manufacturers use to quality control columns render little information about a column’s selectivity and performance characteristics for peptides. The Canadian corporation, SPI, produces a well characterized yet challenging peptide mixture that is very useful for quantifying HPLC column performance for peptides. The difficult to separate first pair of peptides are resolved to near base line on Advantage HIGHLOAD C18 columns. Many so-called peptide analysis columns are unable to separate this pair.
